EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.5.1.77 | 30 |
- |
purified recombinant His-tagged enzyme, half-life is about 15 h |
756214 |
| 3.5.1.77 | 40 |
- |
pH 6.0-9.0, 40 min, less than 10% loss of activity |
31894 |
| 3.5.1.77 | 40 |
- |
pH 7.0, 20 min, stable below |
31891 |
| 3.5.1.77 | 40 |
- |
purified recombinant His-tagged enzyme, half-life is below 30 min, inactivation after about 7 h |
756214 |
| 3.5.1.77 | 40 |
- |
stable for at least 24 h |
31899 |
| 3.5.1.77 | 50 |
- |
1 h, stable up to |
31894 |
| 3.5.1.77 | 50 |
- |
half-life: 8.1 h (wild-type enzyme), 20.4 h (mutant enzyme P295C/F304C), 16.2 h (mutant enzyme P295C/F304C), 8 h (mutant enzyme P178C), 9.0 h (mutant enzyme A222C) |
669884 |
| 3.5.1.77 | 50 |
- |
pH 7.0, 30 min, stable up to |
667845 |
| 3.5.1.77 | 50 |
- |
purified recombinant His-tagged enzyme, inactivation within 1 h |
756214 |
| 3.5.1.77 | 55 |
- |
1 h, 25% loss of activity |
31894 |
