EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.5.1.2 | -999 |
- |
10% NaCl enhances thermal stability of glutaminase I |
209026 |
3.5.1.2 | 4 |
- |
low level of activity after partial purification at 4°C in absence of stabilizing ligands, warming at 24°C results in 2fold to 5fold increase in activity |
209021 |
3.5.1.2 | 20 |
50 |
the enzyme retains relative stability up to 95% from 20 to 35°C and 50% from 40 to 50°C for 30 min. The stability of enzyme decreases sharply at higher temperature |
733133 |
3.5.1.2 | 30 |
- |
pH 7.5, 10 min, stable in absence of NaCl |
209026 |
3.5.1.2 | 30 |
- |
stable for at least 2 h |
752635 |
3.5.1.2 | 35 |
- |
30 min, pH 7.5, the enzyme from strain RIB40 is stable |
669542 |
3.5.1.2 | 37 |
- |
pH 7.0, 93% loss of activity, stable in presence of 1 mM EDTA, 20% loss of activity in presence of 1 mM EGTA |
209022 |
3.5.1.2 | 40 |
- |
pH 7.5, 10 min, 10% NaCl, glutaminase I and II stable up to |
209026 |
3.5.1.2 | 40 |
70 |
free and immobilized enzymes retain 85% of their activity during a 45 min of incubation at 40°C and 50°C. Both L-glutaminase enzyme lose about 5% of their original activity at 70°C after 45 min |
733233 |
3.5.1.2 | 50 |
- |
1 h, 91% residual activity |
752531 |