EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.4.2.1 | -999 |
- |
adenine selectively protects the adenosine phosphorylase against heat inactivation, no effect on inosine-guanosine phosphorylase. Hypoxanthine selectively protects inosine-guanosine phosphorylase activity, no effect on adenosine phosphorylase |
489691 |
| 2.4.2.1 | -999 |
- |
three pairs of intrasubunit disulfide bridges play a role in the stability of the enzyme against thermal inactivation |
673591 |
| 2.4.2.1 | 20 |
80 |
purified recombinant enzyme, pH 7.0, 1 h. The enzyme is stable retaining over 90% of the initial activity at up to 37°C at pH 7.0, the activity is completely abolished at 60°C |
759339 |
| 2.4.2.1 | 30 |
- |
1 month, less than 20% loss of activity |
489668 |
| 2.4.2.1 | 37 |
- |
10 min, pH 5 or 8, wild-type enzyme and mutant enzyme N243S show no detectable loss in activity, mutant enzyme N243D/K244Q shows 5% loss at pH 8 and 11% loss at pH 5 |
489706 |
| 2.4.2.1 | 37 |
- |
30 min, stable up to |
686625 |
| 2.4.2.1 | 40 |
- |
stable up to |
723802 |
| 2.4.2.1 | 42 |
- |
30 min, 26% loss of activity |
686625 |
| 2.4.2.1 | 42 |
- |
stable up to |
489703 |
| 2.4.2.1 | 45 |
- |
half-life at pH 6-7: 8-9 min. Half-life at pH 7-8: 5-7 min. At pH 6.5 in presence of 5 mg/ml bovine serum albumin half-life is 17 min |
489693 |
