EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   2.4.1.207 | -999 |
- |
boiling inactivates |
636810 |
| 2.4.1.207 | -999 |
- |
inactivation within 10 min at 100°C |
759991 |
| 2.4.1.207 | -999 |
- |
isoenzymes are heat-labile, none of the isoenzymes is particularly cold-tolerant |
636827 |
| 2.4.1.207 | 0 |
37 |
isozyme XTH14 shows high activity at 37°C, and at least 80% of this activity is maintained down to 20°C. At 4°C the protein still displays more than 60% of ist activity at 37°C. The activity abruptly decreases to about 30% at 0°C. Isozyme XTH26 shows high activity at 37°C, and at least 50% of this activity is maintained down to 20°C. At 4°C the protein still displays more than 30% of ist activity at 37°C, while the activity abruptly decreases to about 15% at 0°C |
704884 |
| 2.4.1.207 | 5 |
- |
all 4 isoenzymes are markedly cold-tolerant retaining activity at 5°C |
636835 |
| 2.4.1.207 | 38 |
50 |
the activity of XETs from nasturtium seeds is stable at 40°C during 2 h (at 38°C during 8 h), but it is completely lost at 50°C |
706280 |
| 2.4.1.207 | 44 |
- |
TCH4 protein: undetectable activity, more heat-sensitive than the total XET activity |
636818 |
| 2.4.1.207 | 52.3 |
- |
unfolding temperature for the recombinant N93S mutant enzyme, slight protection by 20% glycerol or sucrose, 0.5 M urea, or NaCl at 350 mM, further destabilization by NaCl at 1 M |
657870 |
| 2.4.1.207 | 52.9 |
- |
unfolding temperature for the recombinant wild-type enzyme, slight protection by 20% glycerol or sucrose, 0.5 M urea, or NaCl at 350 mM, further destabilization by NaCl at 1 M |
657870 |
| 2.4.1.207 | 95 |
- |
about 25% of isozyme XET6 activity survives incubation at 95°C for 1 min |
703656 |
