EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
2.4.1.17 | -999 |
- |
isoform UGT1A9 is rather stable against heat treatment. Molecular dynamics simulation of the structures revealed that Arg42, Leu173, Leu219, His221 and Arg222 as well as residues Lys91, Ala92, Tyr106, Gly111, Tyr113, Asp115, and Glu241 are responsible for the thermal stability |
703427 |
2.4.1.17 | -60 |
- |
as pellets with a 1.15% KCl overlay, no apparent loss of activity for three months |
488725 |
2.4.1.17 | -20 |
- |
rapid loss of activity |
488698 |
2.4.1.17 | 0 |
- |
mixtures of enzyme and lipid, under stream of argon, stable at least 8 hours |
488726 |
2.4.1.17 | 24 |
- |
1 d, more than 50% loss of activity |
488722 |
2.4.1.17 | 30 |
- |
15 min, absence of substrates, slight inactivation |
488742 |
2.4.1.17 | 35 |
- |
reversible change from active to inactive form, this transition temperature is lowered by cholesterol in phosphatidylcholine bilayers |
488705 |
2.4.1.17 | 37 |
- |
inactivation in 15 min |
488730, 488737 |
2.4.1.17 | 37 |
- |
stable up to |
488755 |
2.4.1.17 | 37 |
- |
wild type UGT1A6 stable for 30 min, mutants inactives after preincubation |
488787 |