EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.3.8.7 | 0 |
37 |
biphasic inactivation, half-lives: 3 h, fast phase, and more than 700 h, slow phase, at 28°C and 2 h, fast phase, and approx. 230 h, slow phase, at 37°C for the wild-type enzyme, the corresponding values for the K304E mutant enzyme are 1.7 h, 100 h at 28°C and 1.25 h and 55 h at 37°C |
391370 |
1.3.8.7 | 25 |
- |
mutant enzyme E376Q is instable at 25°C |
685133 |
1.3.8.7 | 37 |
- |
60 min, bout 20% resudal activity |
743035 |
1.3.8.7 | 41 |
- |
30 min, bout 20% resudal activity |
743035 |
1.3.8.7 | 41 |
- |
wild-type enzyme: almost no loss of activity after 1 h at 41°C, T168A mutant enzyme: complete inactivation after 20 min at 41°C |
391373 |
1.3.8.7 | 42 |
- |
decreased stability of mutant enzymes Y42H and K304E above 42°C compared to wild-type enzyme |
655491 |
1.3.8.7 | 44.5 |
- |
melting temperature, mutant Y158H |
743657 |
1.3.8.7 | 50 |
- |
melting temperature, mutant R206C |
743657 |
1.3.8.7 | 52 |
- |
melting temperature, mutant A52V |
743657 |
1.3.8.7 | 52 |
- |
melting temperature, mutant R413S |
743657 |