EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   1.13.12.5 | -999 |
- |
at 37°C reduced activity compared with remaining activity at 25°C |
695537 |
| 1.13.12.5 | 4 |
50 |
at 50°C after 30 min incubation time only 20% residual activity, at 40°C after 1 h incubation time complete loss of activity |
700221 |
| 1.13.12.5 | 30 |
- |
Rluc remaining activity in the absence and presence of 0.6 M and 1.2 M of trehalose, and 1.2 M of sucrose at 30°C is 15%, about 50%, around 100%, and almost 60%, respectively, after 60 min |
745101 |
| 1.13.12.5 | 30 |
52 |
the F116L/I137V mutant shows a transition starting at 30°C, which is 5°C lower than the wild-type, and ending at 52°C, which is 5°C higher than the wild type. N178D mutant has almost the same denaturation profile |
746365 |
| 1.13.12.5 | 35 |
47 |
purified recombinant His-tagged wild-type RLuc retains full activity up to 35°C and is inactivated at 47°C |
746365 |
| 1.13.12.5 | 37 |
- |
C-SRLuc8 and SRLuc8 start to collapse at temperatures close to 37°C |
744428 |
| 1.13.12.5 | 37 |
- |
mutations Tat229C/A-230 and Tat 229-230 retained higher activity than other mutations |
695537 |
| 1.13.12.5 | 37 |
- |
pH 7.8, 60 min, 4 and 10fold increases in recombinant His-tagged engineered super RLuc8 thermostability are observed at 37°C after 10 and 20 min incubation, respectively, in comparison to the recombinant His-tagged wild-type enzyme. While the wild-type enzyme is completely inactivated after 30 min, the mutant enzyme retains about 30% of its initial activity |
744813 |
| 1.13.12.5 | 39 |
- |
temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme F149S |
674481 |
| 1.13.12.5 | 40 |
- |
in the absence and presence of 0.6 M of trehalose, the remaining activity is only about 2% and 6%, respectively, although it increases about 70fold upon incubation of the enzyme solution in a buffer containing 1.2 M of trehalose. It also seems that the remaining activity in the presence of 1.2 M of sucrose in the first 10 min is slightly better than in its absence, and a little activity is seen even after 60 min |
745101 |
