EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.5.5.5 | 30 |
- |
10 h, about 50% loss of activity |
756265 |
| 3.5.5.5 | 40 |
- |
3 h, about 50% loss of activity |
756265 |
| 3.5.5.5 | 35 |
- |
30 min, in 10 mM potassium phosphate buffer, pH 7.5, 25% loss of activity |
32503 |
| 3.5.5.5 | 40 |
- |
30 min, in 10 mM potassium phosphate buffer, pH 7.5, inactivation |
32503 |
| 3.5.5.5 | 20 |
- |
40 h, about 50% loss of activity |
756265 |
| 3.5.5.5 | 30 |
- |
6 h, 87% residual activity |
667345 |
| 3.5.5.5 | 45 |
- |
and above, purified recombinant enzyme, pH 8.0, 1 h, no activity remaining |
718670 |
| 3.5.5.5 | 30 |
- |
and below, 30 min, stable in 10 mM potassium phosphate buffer, pH 7.5 |
32503 |
| 3.5.5.5 | 65 |
- |
complete loss of activity |
670163 |
| 3.5.5.5 | 15 |
55 |
exposure of the enzyme at 15, 25, and 35°C for 5 h causes 8%, 15%, and 28% decrease in arylacetonitrilase activity, respectively. A further increase in the temperature to 45 and 55°C results in 58% and 90% decrease in activity, respectively. The half-life of this enzyme at 15, 25, 35, 45, 50, and 55°C is 31 h, 16 h, 9 h, 4 h 30 min, 3 h 20 min, and 2 h 40 min, respectively |
733546 |
