EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    3.5.4.5 | 15 |
75 |
wild-type enzyme and mutant R68Q are stable, while the stability of mutant Y60G is decreased above 65°C |
689935 |
| 3.5.4.5 | 50 |
- |
15 min, absence of dithiothreitol, 44% residual activity |
650635 |
| 3.5.4.5 | 50 |
- |
relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C |
209649 |
| 3.5.4.5 | 60 |
- |
relatively labile to thermal treatment, complete inactivation by incubation for 30 min |
209660 |
| 3.5.4.5 | 65 |
- |
stable up to |
657220 |
| 3.5.4.5 | 70 |
90 |
the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C |
712721 |
| 3.5.4.5 | 72 |
- |
30 min, irreversible denaturation |
657220 |
| 3.5.4.5 | 80 |
- |
stable for at least 60 min |
669137 |
| 3.5.4.5 | 90 |
- |
5 min, 33% loss of activity |
669137 |
| 3.5.4.5 | 100 |
- |
1 h, 1 mM dithiothreitol, 48% residual activity |
650635 |
