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Results 1 - 10 of 10
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Reference
15
75
wild-type enzyme and mutant R68Q are stable, while the stability of mutant Y60G is decreased above 65°C
50
-
15 min, absence of dithiothreitol, 44% residual activity
50
-
relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C
60
-
relatively labile to thermal treatment, complete inactivation by incubation for 30 min
65
-
stable up to
70
90
the CDA activity of genes SH1067 and SH1077 remains constant for 3 h at 70°C, the half-time of residual activities of SH1067 and SH1077 increases up to 200% as compared with that of gene T53 at 80°C, SH1067 shows over 200% increased half-time than that of T53, although 120 min later, no activity remains at 90°C. SH2426 shows 150% increased half-time than that of T53 at 70°C. SH2429 shows over 50-70% decreased thermostability than that of T53 at 70°C, residual activity disappears after 10 min at over 80°C
72
-
30 min, irreversible denaturation
80
-
stable for at least 60 min
90
-
5 min, 33% loss of activity
100
-
15 min, 1 mM dithiothreitol, 80% residual activity; 1 h, 1 mM dithiothreitol, 48% residual activity; 1 h, presence of D,L-lipoic acid, pH 7.0, 75% residual activity; 2 h, 50 mM dithiothreitol, 56% residual activity
Results 1 - 10 of 10