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EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Reference
-999
-
enzyme unfolding experiments using recombinant MBP-tagged enzyme in 20 mM Hepes, pH 8.0, at 60-95C, the MBP-tagged enzyme unfolds at lower temperature around 56C due to unfolding of the MBP tag
45
-
50% loss of activity of wild-type PutA86-669 after 1 h, 50% loss of of L432P mutant form of PutA86-669 after 8 min
70
-
10 min, about 90% of the activity remains
70
-
10 min, fully active
70
-
the alpha4beta4-type PDHbeta retains more than 80% of its activity after heating at 70C for 20 min; the alphabetagammadelta-type PDHbeta nearly loses all of its activity during incubation at 70C for 20 min
70
-
the alpha4beta4-type PDHbeta retains more than 80% of its activity after heating at 70C for 20 min; the alphabetagammadelta-type PDHbeta retains 100% of activity during incubation at 70C for 20 min
70
-
the alpha4beta4-type PDHbeta retains more than 80% of its activity after heating at 70C for 20 min; the alphabetagammadelta-type PDHbeta retains 76% of activity during incubation at 70C for 20 min
70
-
the alphabetagammadelta-type PDHbeta retains 68% of activity during incubation at 70C for 20 min
80
-
120 min, 40% of activity remains
90
-
the enzyme is stable for 120 min at 90C
Results 1 - 10 of 11 > >>