EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.1.71 | -999 |
- |
thermal denaturation is fully irreversible and follows first-order kinetics |
726699 |
1.1.1.71 | 4 |
60 |
the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C |
737536 |
1.1.1.71 | 8 |
25 |
the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h) |
740200 |
1.1.1.71 | 30 |
- |
no loss of activity after 15 min, complete loss of activity at 60°C |
287280 |
1.1.1.71 | 41 |
- |
melting temperature T1/2 of wild-type enzyme ADHA |
760769 |
1.1.1.71 | 48 |
- |
melting temperature T1/2 of mutant ADHA-0278 |
760769 |
1.1.1.71 | 53 |
- |
melting temperature T1/2 of mutant ADHA-0279 |
760769 |
1.1.1.71 | 65 |
- |
melting temperature T1/2 of mutant ADHA-0391 |
760769 |
1.1.1.71 | 66 |
- |
melting temperature T1/2 of mutant ADHA-0384 |
760769 |
1.1.1.71 | 67 |
- |
melting temperature T1/2 of mutant ADHA-0398 and of mutant ADHA-0381 |
760769 |