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EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.4.1.3122 - room temperature is recommended in the absence of Triton X-100, because the proteins tend to precipitate at 37°C. In the presence of Triton X-100 (1% v/v) the temperature can be increased without precipitation to 37°C (Mg2+ as activator), or even to 45°C (Mn2+ as activator) 731742
Display the word mapDisplay the reaction diagram Show all sequences 4.4.1.3137 - room temperature is recommended in the absence of Triton X-100, because the proteins tend to precipitate at 37°C. In the presence of Triton X-100 (1% v/v) the temperature can be increased without precipitation to 37°C with Mg2+ as activator 731742
Display the word mapDisplay the reaction diagram Show all sequences 4.4.1.3145 - room temperature is recommended in the absence of Triton X-100, because the proteins tend to precipitate at 37°C. In the presence of Triton X-100 (1% v/v) the temperature can be increased without precipitation to 45°C with Mn2+ as activator 731742
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