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EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Reference
3.4.21.66
-999
-
the temperature optimum increases with incresing MW of substrate
29563
3.4.21.66
40
70
substrate N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
731179
3.4.21.66
50
-
-
753214
3.4.21.66
60
-
-
732254
3.4.21.66
60
-
assay at
732871
3.4.21.66
60
-
esterolysis
29563
,
29565
3.4.21.66
60
-
free enzyme
753026
3.4.21.66
70
-
immobilized enzyme on chitosan, silica gel 100-200, alginate and glutaraldehyde activated chitosan and nitrocellulose membrane
753026
3.4.21.66
75
-
amino acid 4-nitroanilides
29565
3.4.21.66
80
-
immobilized enzyme on amberlite IR 120, DEAE Sephadex A50, and polyacrylamide
753026
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