EC Number   |
|---|
   7.4.2.3 | chimeric Hsp70s overexpressed in BB1553 cells, mtHsp70 overexpressed in the yeast strain YKN3B |
| 7.4.2.3 | cloned in Escherichia coli, expression of ATPase and co-expression of the Hep1 in plasmids containing Ssc1 constructs |
| 7.4.2.3 | cpHsc70-1 and cpHsc70-2 cDNAs are PCR amplified and cloned into NdeI and XhoI sites of pET28a and introduced into Escherichia coli BL21(DE3) |
| 7.4.2.3 | expression of mtHsp70 and its isolated ATPase domain, as well as the ATPase fused to the peptide-binding domain of HscA, in Escherichia coli as insoluble proteins |
| 7.4.2.3 | for purification of the His-tagged human mtHsp70, coexpression is carried out with yeast Hsp70 escort protein Hep in Escherichia coli BL21(DE3) |
| 7.4.2.3 | HEK-293 cells are transiently transfected with pHep-EGFP, expression of C-terminally His-tagged Hsp70 without its N-terminal mitochondrial targeting sequence in Escherichia coli, the enzyme is insoluble in absence of human escort protein Hep, also as isolated ATPase domain and a chimera having this domain fused to the peptide-binding domain of HscA, a soluble monomeric chaperone |
| 7.4.2.3 | recombinant production of the C-terminally His-tagged enzyme by Triticum aestivum germ lysates, co-expression with the proteins of the interactome of the TIM23 core complex |
| 7.4.2.3 | SSC1 expression in Saccharomyces cerevisiae |
| 7.4.2.3 | the sequence encoding human Hsc70 is inserted into the pProExHTa vector |
| 7.4.2.3 | the sequence encoding rat Hsc70 is inserted into the pET11a vector |
