EC Number |
---|
6.4.1.4 | - |
6.4.1.4 | cDNA cloned and expressed in Escherichia coli |
6.4.1.4 | decarboxylase operon cloned and sequenced, overproduced in Escherichia coli |
6.4.1.4 | expressed in Escherichia coli |
6.4.1.4 | expression as fusion proteins in human fibroblasts |
6.4.1.4 | expression of His-tagged MCCase in Escherichia coli strain BL21 |
6.4.1.4 | MCCase subunits are co-expressed in Escherichia coli in the same plasmid and purified in one step by affinity chromatography using the LiuD-His tag protein interacting with the LiuB-S tag recombinant protein |
6.4.1.4 | reverse transcriptase PCR |
6.4.1.4 | singlecopy gene encoding MCC-A subunit isolated nd characterized |
6.4.1.4 | the alpha and beta subunits of PaMCC are co-expressed in Escherichia coli as His-tagged fusion proteins |