EC Number   |
|---|
    6.3.5.2 | - |
| 6.3.5.2 | cloning of ATPP/DD (a construct that contains the ATP-pyrophosphatase domain as well as the predicted dimerization domain), expression and purification of the corresponding protein, as both a His-tagged fusion protein (His-ATPP/DD) and as a non-fusion protein (NF-ATPP/DD). Both His-ATPP/DD and NF-ATPP/DD are active proteins, capable of catalyzing the conversion of XMP to GMP using exogenously added ammonia |
| 6.3.5.2 | construction of a plasmid for high level expression of XMP aminase in Escherichia coli |
| 6.3.5.2 | expressed in Escherichia coli BL21(DE3) cells |
| 6.3.5.2 | expressed in Escherichia coli BL21(DE3) gold pRARE cells |
| 6.3.5.2 | expressed in Escherichia coli DH5alpha cells |
| 6.3.5.2 | expression in Escherichia coli |
| 6.3.5.2 | gene guaA, the guaAB operon of Borrelia burgdorferi is present on plasmid cp26 |
| 6.3.5.2 | genes ph1346 and ph1347, genetic structure of GMPS, overview. Expressionin Escherichia coli strain BL21(DE3) |
| 6.3.5.2 | isolation of the gene |
