EC Number   |
|---|
    6.3.4.15 | - |
| 6.3.4.15 | coding region of birA biotin-protein ligase gene cloned from genomic DNA by PCR, verfied by sequencing and expressed in mouse erythroleukemic cells |
| 6.3.4.15 | codon-optimized expression in Escherichia coli |
| 6.3.4.15 | construction and expression of the C-terminal 87 amino acids of biotin carboxyl carrier protein, i.e. BCCP-87. cross-species activity of BPLs permitts functional cloning of the cDNA for yeast BPL through genetic complementation with a conditional lethal birA- strain of Escherichia coli |
| 6.3.4.15 | cross-species activity of BPLs permitts functional cloning of the cDNA for human BPL through genetic complementation with a conditional lethal birA? strain of Escherichia coli |
| 6.3.4.15 | cross-species activity of BPLs permitts functional cloning of the cDNA for yeast BPL through genetic complementation with a conditional lethal birA? strain of Escherichia coli |
| 6.3.4.15 | DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21 |
| 6.3.4.15 | doxycycline-regulated expression of a secreted variant of Escherichia coli biotin ligase BirA with target proteins containing a 13-residue biotin acceptor peptide appended to their extracellular domain, co-expression with three different G-protein coupled receptors, i.e. protease-activated receptors 1 and 2, and the platelet ADP receptor, P2Y12, which become biotinylated, in CHO-K1 Tet-On cells on the cell surface, overview |
| 6.3.4.15 | expressed as thioredoxin- and maltose binding protein-fusion proteins in Escherichia coli BL21(DE3) cells |
| 6.3.4.15 | expressed in Escherichia coli BL21 cells |
