EC Number   |
|---|
    5.5.1.18 | - |
| 5.5.1.18 | a lycopene beta-cyclase/lycopene epsilon-cyclase/light-harvesting complex-fusion protein is expressed in Escherichia coli BL21(DE3) cells |
| 5.5.1.18 | expressed in Arabidopsis thaliana |
| 5.5.1.18 | expression in Escherichia coli |
| 5.5.1.18 | expression in Escherichia coli results in the simultaneous accumulation of alpha- beta-, delta-, and epsilon-carotene |
| 5.5.1.18 | gene IbLCYe, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, 4029 bp and 5 introns and 6 exons deduced, real-time quantitative PCR expression analysis, heterologous expression in transgenic Nicotiana tabacum cv. Wisconsin 38 using Agrobacterium tumefaciens strain EHA105 transfection leads to accumulation of significantly increased beta-carotene compared to the untransformed control plants |
| 5.5.1.18 | gene Lcy-e, expression in tomato fruits under control of the 35S CaMV promoter in cotyledons via Agrobacterium tumefaciens-mediated transformation, the transgenic plants show increased lutein levels, quantitative real-time quantitative PCR expression analysis |
| 5.5.1.18 | gene MpLCYe, DNA and amino acid sequence determination and analysis |
| 5.5.1.18 | recombinant expression in Escherichia coli strain BL21(DE3) of diverse constructs of the enzyme in fusion with GST,HNGW, HXGW, His3 or His6 tags, and in fusion with maltose-binding protein tags, 3C protease cleavage site, N-utilizing substance A, or thioredoxin, for in vivo complementation, the plasmids are transformed into Escherichia coli strain JM109 containing the plasmid pFarbeR enabling lycopene synthesis |
| 5.5.1.18 | single copy gene Czlcy-e, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, heterologous expression and complementation in Escherichia coli strain BL21(DE3) |
