EC Number |
---|
5.4.99.11 | - |
5.4.99.11 | cloning and expression in Escherichia coli strain DH10B, expression on cell surface of Saccharomyces cerevisiae strain FMB-1 |
5.4.99.11 | cloning of the PdSIase gene into the expression vector pET28a, functional recombinant expression in Yarrowia lipolytica strain CGMCC7326 on the cell surface using the cell wall protein Pir1 as an anchor protein, and recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) |
5.4.99.11 | DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His6-tagged enzyme in Escherichia coli |
5.4.99.11 | expressed in Escherichia coli BL21(DE3) cells |
5.4.99.11 | expressed in Escherichia coli JM109 cells |
5.4.99.11 | expressed in sugarcane (Saccharum sp.) cultivar Q117 embryogenic callus |
5.4.99.11 | expression in Escherichia coli |
5.4.99.11 | expression in Escherichia coli, with His-tag |
5.4.99.11 | expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |