EC Number   |
|---|
   5.1.3.8 | - |
| 5.1.3.8 | 2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production |
| 5.1.3.8 | construction of a series of chimeric enzymes successively replacing the three domains of the human enzyme - N-terminal, middle, and C-terminal - with the corresponding domains of the rat enzyme. Chimeras are expressed in Escherichia coli JM109 under the control of the Taq promoter |
| 5.1.3.8 | construction of several C-terminal deletion and multi-cysteine/serine mutants and expression in Escherichia coli |
| 5.1.3.8 | expressed in Escherichia coli JM109 under the transcriptional control of taq promoter |
| 5.1.3.8 | expression in Escherichia coli |
| 5.1.3.8 | expression in Jurkat cells |
| 5.1.3.8 | expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies, subcloning in Escherichia coli strain DH5alpha |
| 5.1.3.8 | expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |
| 5.1.3.8 | expression of wild-type enzyme and mutant enzymes C41S, C66S, C104S, C125S, C210S, C239S, C302S, C380S, C386S and C390S in Escherichia coli |
