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Results 1 - 10 of 10
EC Number Cloned (Commentary) Reference
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7- 2141, 2155, 663354
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7DAP epimerase mutants C73S and C217S from Haemophilus influenzae are cloned and purified 671810
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7expressed in Escherichia coli BL21 (DE3) 701554
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7expression in Escherichia coli BL21(DE3) 660601
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7gene dapF, overexpression of N-terminally His6-tagged enzyme, cloned with a a thrombin cleavage site, in Escherichia coli strain SoluBL21 726632
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7gene dapF, recombinant expression of seleno-methionine substituted wild-type and mutant enzymes in Escherichia coli strain B834(DE3) 749346
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7gene dapF, sequence comparisons and phylogenetic analysis, Chlamydia trachomatis dapF restores growth in an Escherichia coli D-glutamate auxotroph strain WM335 also lacking endogenaous gene dapF, expression under the control of an arabinose-inducible promoter, the recombinant enzyme is capable of both DAP epimerase and D-glut racemase activities, DapFCT is also capable of racemizing D-Glu to L-Glu 748536
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7gene dapF1, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS/pET15b 728008
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7recombinant protein is expressed as His-tegged enzyme in BL21(DE3) Escherichia coli cells 701470
Show all pathways known for 5.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.7since previous attempts to express the diaminopimelate epimerase gene dapF of Mycobacterium tuberculosis in Escherichia coli results in undetectable enzyme yields a recombinant DapF protein is expressed in Escherichia coli consisting of silent mutation of the first 10 codons of the open reading frame in an attempt to reduce the formation of secondary structures that occur near the 5' end of the mRNA and inhibit translation. This significantly increases the yield of the enzyme 673738
Results 1 - 10 of 10