EC Number   |
|---|
    5.1.1.7 | - |
| 5.1.1.7 | DAP epimerase mutants C73S and C217S from Haemophilus influenzae are cloned and purified |
| 5.1.1.7 | expressed in Escherichia coli BL21 (DE3) |
| 5.1.1.7 | expression in Escherichia coli BL21(DE3) |
| 5.1.1.7 | gene dapF, overexpression of N-terminally His6-tagged enzyme, cloned with a a thrombin cleavage site, in Escherichia coli strain SoluBL21 |
| 5.1.1.7 | gene dapF, recombinant expression of seleno-methionine substituted wild-type and mutant enzymes in Escherichia coli strain B834(DE3) |
| 5.1.1.7 | gene dapF, sequence comparisons and phylogenetic analysis, Chlamydia trachomatis dapF restores growth in an Escherichia coli D-glutamate auxotroph strain WM335 also lacking endogenaous gene dapF, expression under the control of an arabinose-inducible promoter, the recombinant enzyme is capable of both DAP epimerase and D-glut racemase activities, DapFCT is also capable of racemizing D-Glu to L-Glu |
| 5.1.1.7 | gene dapF1, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS/pET15b |
| 5.1.1.7 | recombinant protein is expressed as His-tegged enzyme in BL21(DE3) Escherichia coli cells |
| 5.1.1.7 | since previous attempts to express the diaminopimelate epimerase gene dapF of Mycobacterium tuberculosis in Escherichia coli results in undetectable enzyme yields a recombinant DapF protein is expressed in Escherichia coli consisting of silent mutation of the first 10 codons of the open reading frame in an attempt to reduce the formation of secondary structures that occur near the 5' end of the mRNA and inhibit translation. This significantly increases the yield of the enzyme |
