EC Number   |
|---|
    4.6.1.12 | - |
| 4.6.1.12 | a 339 bp core fragment and full-length DNA of MECPS amplified and subcloned into pGEM T-easy vector. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS |
| 4.6.1.12 | amplification product digested with BamHI and PstI and ligated into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue |
| 4.6.1.12 | cloned into pET17b for expression of the native polypeptide in Escherichia coli strain BL21 |
| 4.6.1.12 | expressed in Escherichia coli |
| 4.6.1.12 | expressed in Escherichia coli strain BL21 (DE3) |
| 4.6.1.12 | expressed in Escherichia coli strain EB370 |
| 4.6.1.12 | expression in Escherichia coli |
| 4.6.1.12 | expression in Escherichia coli strain NMW26 and Arabidopsis thaliana |
| 4.6.1.12 | final amplification product digested with the restriction endonucleases BamHI and SalI and cloned into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue |
