EC Number |
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4.2.99.B1 | codon-optimized gene POLB, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli |
4.2.99.B1 | expressed in Escherichia coli and purified |
4.2.99.B1 | expressed in Escherichia coli BL21(DE3) via electroporation, lacking the 9-amino acid mitochondrial targeting signal sequence |
4.2.99.B1 | expressed in Escherichia coli strain BL21 (DE3) competent cells |
4.2.99.B1 | expressed in MB36.3 cell, wild-type, SV40 T-Ag transformed cell line haboring the lambda phage transgene lambdaLIZ |
4.2.99.B1 | expressed in Spodoptera friguperda |
4.2.99.B1 | expresses in Escherichia coli M15 (pREP4). The protein aggregates as inclusion bodies, solubilized in 5 M guanidinium hydrochloride and 20 mM inidazole-HCl, pH 8.0 and purified |
4.2.99.B1 | expression of mutant enzymes by coupled in vitro transcription and translation from a modified DNA template containing a TAG codon at the position corresponding to Lys72 |
4.2.99.B1 | gene Rev1, recombinant expression of His-tagged full-length enzyme in in Escherichia coli |
4.2.99.B1 | MB38DELTA4, beta-pol null, sensitive to the methylating agent methylmethanesulfonate, SV40 T-Ag transformed cell line haboring the lambda phage transgene lambdaLIZ |