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EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.2.99.23expressed in Escherichia coli Rosetta 2 (DE3) cells
Display the word mapDisplay the reaction diagram Show all sequences 4.2.99.23gene TgTCEB1, recombinant expression of the wild-type enzyme in Escherichia coli strain Rosetta 2 (DE3)/pET28a
Display the word mapDisplay the reaction diagram Show all sequences 4.2.99.23gene TgTCEB1, sequence comparisons and unrooted phylogenetic tree, functional recombinant expression of the mature dimeric enzyme in Escherichia coli. Although the recombinant enzyme is functionally expressed as N-terminal His-tagged protein, removal of the vector-derived sequence including His-tag by protease treatment almost doubles its activity, allowing for accurate assessment of enzyme characteristics. Recombinant transient expression of GFP-tagged N-terminal transit peptides of TCEB in onion epidermal cell plastids
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