EC Number   |
|---|
   4.2.3.57 | codon-optimized QHS1 gene, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH |
| 4.2.3.57 | expressed in Escherichia coli BL21 (DH3) pLysS cells |
| 4.2.3.57 | expressed in Escherichia coli BL21 cells |
| 4.2.3.57 | expressed in Escherichia coli BL21(DE3) cells |
| 4.2.3.57 | expressed in Escherichia coli BL21, Rosetta and RIPL strains and in Arabidopsis thaliana |
| 4.2.3.57 | expressed in Escherichia coli strain YJM59 |
| 4.2.3.57 | gene OkBCS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative RT-PCR expression analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli TOP10 cells, transient overexpression in Ocimum kilimandscharicum via Agrobacterium tumefaciens strain GV3101 mediated transfection |
| 4.2.3.57 | gene QHS1, the optimized QHS1 gene is expressed in Escherichia coli strain YJM63 and BL21(DE3), coexpression with genes ACS, GPPS2, and AACS to construct a beta-caryophyllene overroduction strain |
| 4.2.3.57 | gene TPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS), illumina transcriptome sequencing of unripe peppercorn identifiying sesquiterpene synthases (sesqui-TPSs), recombinant expression of His-tagged enzyme TPS1 in Escherichia coli strain Rosetta 2(DE3) |
| 4.2.3.57 | gene TPS1, sequence comparisons and phylogenetic tree, isozyme TPS1 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) |
