EC Number   |
|---|
    4.2.3.4 | - |
| 4.2.3.4 | expression in Escherichia coli |
| 4.2.3.4 | expression in Escherichia coli BL21 |
| 4.2.3.4 | into the pET23a+ vector for expression in Escherichia coli BL21DE3 cells |
| 4.2.3.4 | overexpressed in Escherichia coli Rosetta DE3. The enzyme is insoluble when expressed in Escherichia coli but is partially solubilised when KCl is included in the cell lysis buffer |
| 4.2.3.4 | overexpression in Escherichia coli |
| 4.2.3.4 | overexpression of the dehydroquinate synthase domain of the pentafunctional AROM protein in Escherichia coli |
| 4.2.3.4 | The aroB gene cloned from Xoo and the corresponding DHQS protein is subsequently overexpressed in Escherichia coli |
| 4.2.3.4 | The aroB gene encoding HpDHQS is amplified from genomic DNA by PCR and inserted into the pQE30 expression vector. The HpDHQS protein is expressed from Escherichia coli JM109 cells transformed with pQE30-HpDHQS |
