4.2.3.32 | gene TPS-LAS, recombinant expression in Saccharomyces cerevisiae strain AM94, a yeast strain engineered for the production of isoprenoids, which contains three chromosomally integrated copies of a degradation stabilized variant of HMG2 and a heterozygous deletion of ERG9, and in strain AM204, each with or without coexpression of yeast protein Erg20p(F96C), a yeast farnesyl diphosphate synthase, overview. Coexpression with different Saccharomyces cerevisiae ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS |
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