EC Number   |
|---|
    4.2.3.17 | - |
| 4.2.3.17 | cDNA encoding taxa-4(5),11(12)-diene synthase is introduced in Nicotiana sylvestris for specific expression in trichome cells. |
| 4.2.3.17 | cDNA is directly cloned into the pGemT-Easy vector, sequenced and cloned into the plant binary transformation vector pBC35. Furthermore it is subcloned into pBluescript, released and ligated into pRD12. The pBC35 and pRD12 constructs are transferred to Agrobacterium tumefaciens and used to transform tomato cotyledons |
| 4.2.3.17 | expressed in Artemisia annua |
| 4.2.3.17 | expressed in Escherichia coli |
| 4.2.3.17 | expressed in Escherichia coli BL21(DE3)RIL CodonPlus cells |
| 4.2.3.17 | expressed in hairy root cultures of Taxus x media variant Hicksii |
| 4.2.3.17 | expressed in Physcomitrella patens |
| 4.2.3.17 | expression in Escherichia coli |
| 4.2.3.17 | Expression of Taxus chinensis taxadiene synthase (expression of a transgene encoding the pseudomature form which lacks the N-terminal 74-amino acid residue plastid-targeting sequence) in Saccharomyces cerevisiae alone does not increase taxadiene levels because of insufficient levels of the universal diterpenoid precursor geranylgeranyl diphosphate. Coexpression of Taxus chinensis taxadiene synthase and geranylgeranyl diphosphate synthase fails to increase levels. Replacement the Taxus chinensis geranylgeranyl diphosphate synthase with its counterpart from Sulfolobus acidocaldarius, which does not compete with steroid synthesis, and codon optimized the Taxus chinensis taxadiene synthase gene to ensure high-level expression, resulting in a increase in taxadiene as well as significant amounts of geranylgeraniol, suggesting taxadiene levels can be increased even further. |
