EC Number   |
|---|
   4.2.3.131 | - |
| 4.2.3.131 | cDNA library screening and miltiradiene DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis |
| 4.2.3.131 | cloning of full-length cDNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant transient Agrobacterium tumefaciens-mediated coexpression of Isodon rubescens gene TPS4, encoding miltiradiene synthase, with Isodon rubescens gene TPS3, encoding (+)-copalyl diphosphate synthase in Nicotiana benthamiana results in production of miltiradiene, while coexpression with Isodon rubescens gene TPS2, encoding ent-copalyl diphosphate synthase, does not lead to product formation |
| 4.2.3.131 | expressed in Saccharomyces cerevisiae |
| 4.2.3.131 | expression in Escherichia coli and Saccharomyces cerevisiae |
| 4.2.3.131 | gene KSL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time PCR enzyme expression analysis, recombinant expression of HA-His-tandem-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL, functional transient recombinant coexpression of Salvia fruticosa (+)-copalyl diphosphate sythase (SfCPS) and Salvia fruticosa ent-kaurene synthase-like (SfKSL) in Nicotiana benthamiana via transfection by Agrobacterium tumefaciens leading to production of miltiradiene in planta, NMR product analysis |
| 4.2.3.131 | gene SKL1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, RoKSL1 and RoKSL2 cluster into a discrete clade, together with other KSL proteins from the Lamiaceae and Solanaceae (NtABS, ShSBS and SlPHS). Functional recombinant expression in Saccharomyces cerevisiae strain AM113, and transient coexpression of RoSKL1 and RoSKL2 in Nicotiana benthamiana leaves |
| 4.2.3.131 | gene SKL2, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, RoKSL1 and RoKSL2 cluster into a discrete clade, together with other KSL proteins from the Lamiaceae and Solanaceae (NtABS, ShSBS and SlPHS). Functional recombinant expression in Saccharomyces cerevisiae strain AM113, and transient coexpression of RoSKL1 and RoSKL2 in Nicotiana benthamiana leaves |
| 4.2.3.131 | gene TwTPS27, sequence comparisons and phylogenetic analysis |
| 4.2.3.131 | TwTPS27v2, phylogenetic analysis and tree, coexpression of TwTPS27v2 with either copalyl diphosphate synthases TwTPS7v2 or TwTPS9v2 in appropriately engineered Saccharomyces cerevisiae strain, or recombinant coexpression in Escherichia coli, the combination with TwTPS7v2 produces miltiradiene, recombinant expression oof GFP-tagged enzyme TwTPS27v2 in Nicotiana benthamiana chloroplasts, quantitative RT-PCR expression analysis |
