EC Number |
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4.2.1.B20 | cloning of another onoceroid synthase gene that shows high sequence identity with a previously identified alpha-onocerin synthase, functional coexpression with pre-alpha-onocerin synthase in yeast leads to the production of tohogenol and serratenediol |
4.2.1.B20 | coexpression of onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module) under control of constitutive J23119 promoter, subcloning in Escherichia coli strain DH5alpha |
4.2.1.B20 | gene BmTC, recombinant coexpression of enzyme BmTC with AaSHC (EC 5.4.99.17) mutant D377C in a squalene epoxidase Erg1-deficient Pichia pastoris strain leading to increased production of (+)-ambrein, recombinant expression of enzyme BmTC mutant D373C |
4.2.1.B20 | recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |