EC Number   |
|---|
    4.2.1.90 | gene LRA3, functional expression in Escherichia coli |
| 4.2.1.90 | gene LRA3, genetic organization, L-rhamnose catabolism genes RHA1, LRA2, LRA3 and LRA4 are organized in a chromosomal gene cluster in Scheffersomyces stipitis. In Scheffersomyces stipitis, another transcription factor than TRC1 is found in the L-rhamnose cluster, FST14. This transcription factor is more up-regulated than TRC1 on L-rhamnose and it might be co-regulating the pectinolytic genes together with TRC1 |
| 4.2.1.90 | gene lraC, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae |
| 4.2.1.90 | gene lraC, DNA and amino acid sequencedetermination and analysis, expression in Saccharomyces cerevisiae |
| 4.2.1.90 | gene lraC, genetic organization, genes lraA, lraB and lraC, as well as the L-rhamnose regulator RhaR are organized in a cluster |
| 4.2.1.90 | gene yfaW, expression of the His6- or His10-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 4.2.1.90 | the genes for L-rhamnose catabolism RHA1, LRA2, LRA3 and LRA4 but not LADH are clustered, genetic organization, phylogenetic analysis, overview. Functional expression of gene LRA3 in Saccharomyces cerevisiae with a strong constitutive promoter. C-terminally his-tagged protein shows no activity and the activity of the N-terminally tagged protein is reduced by about 95%, so the enzyme is expressed without tagand not purified |
