EC Number   |
|---|
    4.2.1.130 | DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells |
| 4.2.1.130 | expression in Nicotiana tabacum cv. Wisconsin. Overexpression of Hsp31 in tobacco imparts robust stress tolerance against diverse biotic stress inducers such as viruses, bacteria and fungi, in addition to tolerance against a range of abiotic stress inducers. During stress, Hsp31 is targeted to mitochondria and induces expression of key stress-related genes |
| 4.2.1.130 | expression of C-terminally GFP-tagged SpDJ-1, expression of wild-type and mutant SpDj-1 proteins in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview |
| 4.2.1.130 | gene DJ1A, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 4.2.1.130 | gene DJ1B, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 4.2.1.130 | gene DJ1C, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 4.2.1.130 | gene DJ1E, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 4.2.1.130 | gene DJ1F, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 4.2.1.130 | gene hchA |
| 4.2.1.130 | gene hsp3101 encoding a Hsp31 protein, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells |
