EC Number   |
|---|
   4.2.1.119 | - |
| 4.2.1.119 | a truncated version (amino acid residues 318-735) of perMFE-2 is expressed in Escherichia coli BL21(DE3) plysS cells as a recombinant protein |
| 4.2.1.119 | AtECH2 contains a peroxisome targeting signal at the C-terminal end, is addressed to the peroxisome in Saccharomyces cerevisiae, and a fusion protein between AtECH2 and a fluorescent protein is targeted to peroxisomes in onion cells. To assess the peroxisomal addressing of AtECH2, a fusion protein between an EYFP at the N terminus and AtECH2 at the C terminus is constructed and expressed under the control of a double cauliflower mosaic virus (CaMV) 35 S viral promoter to allow transient expression of the fusion protein in onion cells following biolistic bombardment. The fluorescence is examined by confocal microscopy after 12 h |
| 4.2.1.119 | expressed in Escherichia coli BL21(DE3) |
| 4.2.1.119 | expression in Escherichia coli BL21 (DE3) |
| 4.2.1.119 | expression in Escherichia coli BL21(DE3) |
| 4.2.1.119 | expression in Pichia pastoris |
| 4.2.1.119 | expression of wild-type and mutant C-terminally His6-tagged MFE2s in Escherichia coli |
| 4.2.1.119 | expression of wild-type and mutant hydratase domains of multifunctional enzyme type 2 hydratase as GFP-tagged protein in Escherichia coli strain JM109 |
| 4.2.1.119 | gene CG3415, Drosophila melanogaster MFE-2 complements a Saccharomyces cerevisiae MFE-2 deletion strain, functional expression of His-tagged MFE-2 in Escherichia coli strain BL21(DE3) pLysS |
