EC Number   |
|---|
    4.2.1.116 | gene hpcd, coexpression of 3-hydroxypropionyl-CoA dehydratase (HPCD) with propionyl-CoA dehydrogenase (PACD) encoded by gene pacd from Candida rugosa and propionate CoA-transferase (PCT) encoded by gene pct from Megasphaera elsdenii in Escherichia coli strain BL21(DE3) under control of the T7 promoter |
| 4.2.1.116 | gene hpcd, recombinant expression of His-tagged enzyme HPCD in Escherichi coli strain Rosetta 2 (DE3), coexpression with 3-hydroxypropionyl-CoA synthetase, acryloyl-CoA reductase, and succinic semialdehyde reductase. Yeast two-hybrid assay for protein interaction analysis of 3-hydroxypropionyl-CoA dehydratase and 3-hydroxypropionyl-CoA synthetase (HPCS-HPCD) |
| 4.2.1.116 | gene Msed_2001, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3)-T1 |
