EC Number   |
|---|
    4.1.3.40 | - |
| 4.1.3.40 | expressed in Corynebacterium glutamicum |
| 4.1.3.40 | expressed in Lithospermum erythrorhizon under the control of the strong (ocs)3mas-promoter |
| 4.1.3.40 | expressed in Pseudomonas putida strain KT2440 |
| 4.1.3.40 | expressed in Schizosaccharomyces pombe |
| 4.1.3.40 | expression in Nicotiana tabacum under control of the constitutive plant promoter. The gene product is targeted into the plastid by fusing it to the sequence for the chloroplast transit peptide of the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Transgenic plants show high chorismate pyruvate-lyase activity and accumulate 4-hydroxybenzoate as beta-glucosides, with the glucose attached to either the hydroxy or the carboxyl function of 4-hydroxybenzoate. The total content of 4-hydroxybenzoate glucosides is approximately 0.52% of dry weight, which exceeds the content of untransformed plants by at least a factor of 1000 |
| 4.1.3.40 | gene sll1797, phylogenetic analysis and tree, expression in Escherichia coli strain BL21AI as GST-tagged protein, and in Escherichia coli strain BW25113 for quantitative expression analysis by real time quantitative PCR. Complementation of ubiquinone-deficient Escherichia coli mutants by catalytic activity of heterologous Sll1797 |
| 4.1.3.40 | production of recombinant enzyme in Escherichia coli and Mycobacterium smegmatis |
| 4.1.3.40 | the ubiC gene is integrated into the chloroplast genome of Nicotiana tabacum under the control of the light-regulated psbA 5'-untranslated region. the limitation for 4-hydroxybenzoate production in nuclear-transformed plants is the activity of chorismate pyruvate-lyase activity. The process becomes substrate-limited only when the enzyme is present at very high levels in the compartment of interest |
