EC Number |
Reference |
---|
4.1.1.31 | - |
4338, 657045, 662338 |
4.1.1.31 | carbon metabolism is analysed in generated transgenic rice plants expressing either PEPC or both phosphoenolpyruvate carboxykinase (PCK) and PEPC: Results suggest that overexpression of PEPC enhances the anaplerotic pathway rather than the initial carbon fixation of the C4-like photosynthetic pathway, and that elevated PEPC activity in combination with PCK activity contributes little to C4-like carbon flow |
682449 |
4.1.1.31 | cloned as a Histag-fusion protein in an Escherichia coli PEPC- (Ppc-) strain |
680658 |
4.1.1.31 | cloned in Escherichia coli DH5alpha. Knock-out as well as over-expression mutants are constructed and characterized. Knocking out phosphoenolpyruvate carboxylase decreases the maximum cell density by 14% and increases the acetate excretion by 7%. Over-expression of phosphoenolpyruvate carboxylase increases the maximum cell dry weight by 91%. No acetate excretion is detected at these increased cell densities |
672744 |
4.1.1.31 | cloning of ppc1 in Escherichia coli |
663094 |
4.1.1.31 | cloning of ppc2 in Escherichia coli |
663094 |
4.1.1.31 | codon-optimized expression in Escherichia coli |
746787 |
4.1.1.31 | expressed in Arabidopsis thaliana |
727209 |
4.1.1.31 | expressed in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter. SvPEPC is capable of efficiently exerting its activity in the plant cell environment so as to cause imbalance between aromatic and non-aromatic amino acid synthesis |
663185 |
4.1.1.31 | expressed in Escherichia coli |
682463, 716881 |