EC Number |
Reference |
---|
3.8.1.8 | - |
247104, 247105, 247107 |
3.8.1.8 | expression in Escherichia coli BL21 (DE3) |
756217 |
3.8.1.8 | expression of holoenzyme and subfragment with catalytic activity in Escherichia coli DH5-alpha |
247102 |
3.8.1.8 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
719887 |
3.8.1.8 | five amino acids within the substrate-binding pocket of AtzA are chosen for randomization yields one variant enzyme with 20fold-greater catalytic efficiency than that of wild-type AtzA |
690560 |
3.8.1.8 | gene atzA, application of a Haematococcus pluvialis-based method to screen AtzA variants from a random mutagenesis library, genotyping, Haematococcus pluvialis strain H1 is used to test atrazine concentrations, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in Inclusion bodies, subcloning in Escherichia coli strain DH5alpha-FT |
733315 |
3.8.1.8 | gene atzA, DNA and amino acid sequence determination and analysis, genotyping of enzymes from eight triazine-degrading Aminobacter aminovorans strains isolated from French agricultural soils recurrently exposed to triazines in 2000. Subcloning in Escherichia coli strain JM109 |
733440 |
3.8.1.8 | gene atzA, expression in Nicotiana tabacum via Agrobacterium tumefaciens strain LBA4404-mediated transformation and expression under control of the CaMV 35S promoter, the transgenic tobacco lines expressing the wild-type atzA from Arthrobacter sp. strain AD1 show resistance to and a strong ability to degrade atrazine |
720662 |
3.8.1.8 | gene atzA, expression in Nicotiana tabacum via Agrobacterium tumefaciens strain LBA4404-mediated transformation and expression under control of the CaMV 35S promoter, the transgenic tobacco lines expressing the wild-type atzA from Pseudomonas sp. strain ADP show resistance to and a strong ability to degrade atrazine |
720662 |
3.8.1.8 | gene atzA, overexpression in Escherichia coli DH5alpha |
654630 |