EC Number   |
|---|
   3.6.4.B8 | expression of RFCS1Ma and RFCS2Ma as N-terminal His6-tagged proteins |
| 3.6.4.B8 | gene holA gene encodes the N-terminal domain (residues 1-140) of the Escherichia coli gamma clamp loader, recombinant His-tagged mini-delta is obtained by expression in Escherichia coli strain BL21(DE3) |
| 3.6.4.B8 | genes dnaX, and holA-D |
| 3.6.4.B8 | recombinant expression of clamp loader complex subunits delta, gamma, delta', chi, and psi |
| 3.6.4.B8 | recombinant expression of replication factor C subunits 2, 3, 4, and 5 with mutated truncated subunit RFC1 |
| 3.6.4.B8 | recombinant expression of small subunit RFCS, and full-length enzyme RFCSL in Escherichia coli strain Rosetta(DE3)/pLysS |
| 3.6.4.B8 | the two SsoRFC polypeptides are coexpressed in Escherichia coli and purified as a complex. The small subunit is individually expressed in Escherichia coli and purified |
