EC Number   |
|---|
   3.6.4.B7 | - |
| 3.6.4.B7 | expression in Escherichia coli |
| 3.6.4.B7 | gene radA DNA and amino acid sequence determination and analysis, recombinant overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 3.6.4.B7 | gene radA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and genotyping of deep sea isolates using the enzyme sequence, overview |
| 3.6.4.B7 | gene radA, highly efficient cis-splicing of PhoRadA intein, recombinant expression of His-tagged enzyme in Escherichia coli strain ER2566 |
| 3.6.4.B7 | gene radA, recombinant coexpression of His-tagged RadA with untagged RadB (UniProt ID D4GZB4), which form the RadAB complex, in Escherichia coli strain Rosetta2 (DE3) with deleted gene arnA. The ratio of RadA to RadB is 4:1, which suggests that the proteins interact with a specific stoichiometry |
| 3.6.4.B7 | gene radA, recombinant enzyme expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)[pLysS] from plasmid pCB1020 under the control of a rifampicin-resistant promoter, PT7 |
| 3.6.4.B7 | gene radA, recombinant expression in Escherichia coli strain BL21 Star (DE3), construction of the plasmid pMIG-RadAi, carrying the Pho RadA intein gene in foreign exteins, is accomplished by inserting the RadA intein sequence with short native exteins into ClaI and SphI sites between sequences coding for MBP and GFP in a pACYCDuet-1 plasmid backbone, splicing of the RadA intein located in the ATPase domain of the hyperthermophilic archaeon Pyrococcus horikoshii is strongly regulated by the native exteins, which lock the intein in an inactive state |
| 3.6.4.B7 | gene radA, recombinant expression in Escherichia coli strain Rosetta |
| 3.6.4.B7 | gene radA, recombinant overexpression in Escherichia coli strain Rosetta |
