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EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.22-
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.22amplified from Streptomyces clavuligerus genomic DNA. Cloned in the pSET152 integration and pIBR25 expression vectors containing the strong ermE promoter. Plasmids are introduced into Streptomyces clavuligerus by protoplast transformation
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.22expressed in Streptomyces clavuligerus strain SMF5704
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.22expression in Streptomyces venezuelae
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.22Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028
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