EC Number |
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3.5.1.77 | expression in Escherichia coli |
3.5.1.77 | gene BJS_06659, sequence comparisons and analysis |
3.5.1.77 | gene hyuD, sequence comparisons and phylogenetic tree, recombinant, mostly soluble His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3) |
3.5.1.77 | into the vector pET28b, a mutagenesis library is created |
3.5.1.77 | mutant A18T/Y30N/K34E is overexpressed in Escherichia coli and up to 80% of it is soluble |
3.5.1.77 | the plasmids pET20bl and pET-Chi are used for expression of the protein in Escherichia coli cells |