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EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 3.5.1.69-
Display the reaction diagram Show all sequences 3.5.1.69recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Display the reaction diagram Show all sequences 3.5.1.69the gene encoding the mature SA_SCD protein, which lacks its 38-residue N-terminal secretion signal sequence and from which the 277-residue C-terminal sequence is deleted, is codon-optimized and expressed in Escherichia coli strain BL21 (DE3) pLysS as Histagged enzyme
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