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Results 1 - 10 of 15 > >>
EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 3.5.1.60expressed in COS-7 cells
Display the reaction diagram Show all sequences 3.5.1.60gene NAAA, genotyping, six splice variants (a1, b1, c1, a2, b2, and c2), whereof four major splice variants (a1, a2, b2, and c2) are identified in a human prostate cancer cell line LNCaP, they are composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively, quantitative PCR expression analysis, method development for individual quantification. Variants a1 and a2 encode the same full-length NAAA protein, which is catalytically active, while b2 and c2 are translated to C-terminally truncated proteins. Recombinant expression in HEK-293 cells. The truncated forms are detected as catalytically inactive precursor proteins, but not as mature forms, expression of N-terminally FLAG-tagged isoform A of human NAAA, corresponding to splice variant a1, in HEK-293 cells. The mature enzyme form loses the N-terminal FLAG tag
Display the reaction diagram Show all sequences 3.5.1.60gene Naaa, recombinant expression in HEK-293 cells
Display the reaction diagram Show all sequences 3.5.1.60gene NAAA, recombinant overexpression in HEK-293 cells
Display the reaction diagram Show all sequences 3.5.1.60gene Naaa, stable transfection and functional recombinant expression in HEK-293 cells
Display the reaction diagram Show all sequences 3.5.1.60recombinant enzyme overexpression in HEK-293 cells
Display the reaction diagram Show all sequences 3.5.1.60recombinant expression in HEK-293 cells
Display the reaction diagram Show all sequences 3.5.1.60recombinant expression of enzyme NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Construct encompasses residues 31-359 of NAAA of mouse enzyme
Display the reaction diagram Show all sequences 3.5.1.60recombinant expression of the C-terminally His6-tagged enzyme in HEK-293 cells
Display the reaction diagram Show all sequences 3.5.1.60recombinant expression of the enzyme in HEK-293 cells
Results 1 - 10 of 15 > >>