EC Number |
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3.4.24.B5 | cloned into pcDNA 3.1/myc-His and subcloned into pLXSN to yield myc-His-tagged MMP-15 |
3.4.24.B5 | expressed in Escherichia coli and in Timp2-/- skin fibroblasts |
3.4.24.B5 | expression of the enzymes catalytic domain in Escherichia coli |
3.4.24.B5 | functional expression of enzyme in HT1080 cells and U251 glioma cells, leading to enhanced levels of pro-matrix metalloprotease-2 |
3.4.24.B5 | gene MMP15, quantitative expression analysis |
3.4.24.B5 | gene MMP15, quantitative RT-PCR enzyme expression analysis |
3.4.24.B5 | gene mmp15, reverse transcription and real-time PCR expression analysis |