EC Number   |
|---|
   3.4.24.72 | chimeric Arg-Gly-Asp-fibrolase is expressed in Escherichia coli BL21(DE3) cells |
| 3.4.24.72 | co-expression of the active signal-less His-tagged enzyme with chaperone DsbC in Escherichia coli cytoplasm, over 90% of the recombinant enzyme is soluble due to DsbC, while the chaperones FkpA and Skp do not have an effect on fibrolase solubility |
| 3.4.24.72 | expressed in Pichia pastoris |
| 3.4.24.72 | expression of the wild-type fibrolase and a truncated enzyme form, alfimeprase |
| 3.4.24.72 | gene alf, high level expression of His-tagged alfimeprase in Pichia pastoris strains of GS115 and KM71 by methanol induction, secretion of the recombinant enzyme to the cell culture medium |
| 3.4.24.72 | production of alfimeprase by recombinant DNA technology |
