EC Number |
---|
3.4.23.36 | - |
3.4.23.36 | DNA and amino acid sequence determination and analysis |
3.4.23.36 | enzyme overexpression in Escherichia coli confers high resistance to globomycin |
3.4.23.36 | expressed in Escherichia coli DH5alpha cells |
3.4.23.36 | expressed in MelJuso cells |
3.4.23.36 | expression of Sp0928 protects Escherichia coli against the Lsp antagonist globomycin |
3.4.23.36 | gene lsp, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli under control of the trp promoter |
3.4.23.36 | ORF Rv1539, gene lspA, from strain H37Rv, DNA and amino acid sequence determination and analysis |
3.4.23.36 | overexpression of Rickettsia typhi lspA in Escherichia coli confers increased globomycin resistance. Recombinant lspA from significantly restores the growth of temperature-sensitive Escherichia coli Y815 at the nonpermissive temperature, supporting its biological activity as SPase II in prolipoprotein processing |
3.4.23.36 | wild-type lsp gene amplified by PCR, cloned into pGDL48 and used to transform Bacillus subtilis 8G5lsp, lacking the lsp gene |