EC Number   |
Reference |
|---|
   3.4.22.B70 | cloning of the His-tagged catalytic domain of SENP1, SENP1c, aa419-aa643, from PC-3 cell cDNA library, cloned into pET28a(+) vector, and expressed in Escherichia coli strain BL21 |
717356 |
| 3.4.22.B70 | crystallization of a complex of SENP1 C603A bound to full-length SUMO-1 and determination of the structure by X-ray crystallography. Two structures of SENP1 C603A in complex with the SUMO-1 precursor and RanGAP1-SUMO-1 |
682081 |
| 3.4.22.B70 | expression as N-terminally His-tagged enzyme in Escherichia coli |
713664 |
| 3.4.22.B70 | expression in Escherichia coli |
715010 |
| 3.4.22.B70 | expression in Escherichia coli BL21 |
714079 |
| 3.4.22.B70 | expression of GFP-tagged SENP2 in HeLa cells |
732481 |
| 3.4.22.B70 | expression of wild-type and mutant enzymes in MEF, 293T, and 3T3-L1 cells, real-time quantitative PCR expression analysis |
732144 |
| 3.4.22.B70 | gene SP1, recombinant expression of GFP-tagged or untagged SENP1 and the catalytically dead enzyme mutant transiently in HeLa cells |
732590 |
| 3.4.22.B70 | overexpression of a catalytically inactive/dominant negative Cys-to-Ser SENP1 mutant |
731577 |
| 3.4.22.B70 | quantitative real-time PCR expression analysis |
732963 |
