EC Number   |
|---|
   3.4.22.68 | expression in Escherichia coli |
| 3.4.22.68 | expression of catalytic domains of human SENP2-(364-589), SENP6-(637-1112), and SENP7-(662-984) and of mutant SENP-7s in Escherichia coli |
| 3.4.22.68 | expression of FLAG-SMT3IP1 in COS-7 cells, coexpression with His6-ubiquitin. Overexpression of SMT3IP1 in cells results in the accumulation of Mdm2 in the nucleolus and increased stability of the p53 protein |
| 3.4.22.68 | expression of SENP1 and SENP2 isozymes in HCT-116 cells |
| 3.4.22.68 | expression of SUMO-S-GroQ fusion protein, i.e. SUMO fused to the Q domain of Drosophila melanogaster Groucho, containing sequences encoding the mature form of SUMO followed by a 6-His tag and a multiple cloning site harbouring the fused protein S-GroQ, co-expression of SUMO-fused SUMO-specific protease Ulp1 |
| 3.4.22.68 | expression of the wild-type full-length PfSENP1 and the catalytic domain of PfSENP1 |
| 3.4.22.68 | expression of wild-type and mutant enzymes, expression of the SBS domain as a SBS-GFP fusion protein |
| 3.4.22.68 | expresssion in Escherichia coli |
| 3.4.22.68 | gene ESD4, DNA and amino acid sequence determination, genotyping |
| 3.4.22.68 | gene ULP1A, DNA and amino acid sequence determination, genotyping, recombinant expression in Arabidopsis thaliana plants and in leaves of Nicotioana bentamiana using transfection via Agrobacterium tumefaciens. An expressed GFP-ELS1 construct has a generally non-nuclear localization, being detectable at membranes and in the cytoplasm |
